Detection of Brucella canis and Leptospira interrogans in Canine Semen by Multiplex Nested PCR
نویسندگان
چکیده
منابع مشابه
Comparing Rapid and Specific Detection of Brucella in Clinical Samples by PCR-ELISA and Multiplex-PCR Method
Background: Rapid diagnosis and differentiation of Brucella is of high importance due to the side effects of antibiotics for the treatment of brucellosis. This study aimed to identify and compare PCR-ELISA as a more accurate diagnositc test with other common molecular and serological tests. Methods: ...
متن کاملCanine Brucellosis: Brucella canis
Canine brucellosis, caused by Brucella canis, is an important cause of reproductive failure, particularly in kennels. This organism causes abortions, stillbirths, epididymitis, orchitis and sperm abnormalities in dogs. Although dogs that have been spayed or neutered do not have reproductive signs, they occasionally develop other conditions such as ocular disease and discospondylitis. B. canis c...
متن کاملCanine Brucellosis Caused by Brucella Canis
Etiology and Epidemiology Canine brucellosis is caused by Brucella canis (B. canis), a rough, small, gram-negative, intracellular bacterium. Other Brucella species, i.e., B. abortus, B. suis, have occasionally caused canine infections but they are not discussed here. The canine brucella was first recognized in 1966 as a cause of abortions and reproductive failures and it has since been recogniz...
متن کاملMULTIPLEX PCR TO DETECT Brucella AND Leptospira SPECIES IN SPIKED MILK SAMPLES
Brucellosis and leptospirosis are zoonotic diseases and cause much economic losses in livestock by causing abortion, stillbirth and infertility. Both organisms are excreted in milk and thus increasing the risk of infecting other animals and human. Early detection of organisms is desirable for effective treatment and control of diseases. In this study, multiplex PCR was attempted to detect Bruce...
متن کاملDetection of Bovine Viral Diarrhea Virus in Bovine Semen Using Nested-PCR
A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2006
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.68.615